Anti-inflammatory potential of ellagic acid, gallic acid and punicalagin A&B isolated from Punica granatum

作者：BenSaad, Lamees A.; Kim, Kah Hwi^*; Quah, Chin Chew; Kim, Wee Ric; Shahimi, Mustafa

来源：BMC Complementary and Alternative Medicine, 2017, 17(1): 47.

DOI：10.1186/s12906-017-1555-0

• Inflammation
• Punica granatum
• Cytotoxicity
• Cytokines
• Ellagic acid
• Gallic acid
• Punicalagin

Background: Punica granatum (pomegranate), an edible fruit originating in the Middle East, has been used as a traditional medicine for treatment of pain and inflammatory conditions such as peptic ulcer. The numerous risks associated with nonsteroidal anti-inflammatory drugs (NSAIDs) for treatment of pain and inflammation give rise to using medicinal herbs as alternative therapies. This study aimed to evaluate the anti-inflammatory effect of isolated compounds from the ethyl acetate (EtOAc) fraction of P. granatum by determination of their inhibitory effects on lipopolysaccharide (LPS), stimulated nitric oxide (NO), prostaglandin E2 (PGE-2), interleukin-6 (IL-6) and cyclooxxgenase-2 (COX-2) release from RAW264.7 cells. @@@ Methods: The compounds ellagic acid, gallic acid and punicalagin A&B were isolated from EtOAc by high performance liquid chromatography (HPLC) and further identified by mass spectrometry (MS). The inhibitory effect of ellagic acid, gallic acid and punicalagin A&B were evaluated on the production of LPS-induced NO by Griess reagent, PGE-2 and IL-6 by immunoassay kit and prostaglandin E2 competitive ELISA kit, and COX-2 by Western blotting. @@@ Results: Ellagic acid, gallic acid and punicalagin A&B potentially inhibited LPS-induced NO, PGE-2 and IL-6 production. @@@ Conclusion: The results indicate that ellagic acid, gallic acid and punicalagin may be the compounds responsible for the anti-inflammatory potential of P. granatum.

• 出版日期2017-1-14
• 单位河北医科大学