Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region

作者：Egawa Takahiro; Koide Yuichiro; Hanaoka Kenjiro; Komatsu Toru; Terai Takuya; Nagano Tetsuo^*

来源：Chemical Communications, 2011, 47(14): 4162-4164.

DOI：10.1039/c1cc00078k

We present a design strategy for fluorescence probes with a high off/on activation ratio in the red wavelength region, based on a novel fluorescein analogue in which the O atom at the 10 position of the xanthene chromophore is replaced with a Si atom. To demonstrate the usefulness of this strategy, we designed and synthesized a red-fluorescent probe for beta-galactosidase, and showed that it works in live HEK293 cells.

• 出版日期2011