A label free fluorescent assay for uracil-DNA glycosylase activity based on the signal amplification of exonuclease I

作者：Ma, Yefei; Zhao, Jingjin; Li, Xuejun; Zhang, Liangliang^*; Zhao, Shulin

来源：RSC Advances, 2015, 5(98): 80871-80874.

DOI：10.1039/c5ra12958c

• REPAIR ENZYME-ACTIVITY
• EXCISION-REPAIR
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We introduced a simple, fast and label free fluorescent assay for uracil-DNA glycosylase activity based on the signal amplification of exonuclease I. This method exhibits a high sensitivity for UDG activity assay with a low detection limit of 0.0070 U mL(-1).

• 出版日期2015
• 单位广西师范大学