Mitochondrial DNA genetic diversity of honey bees, Apis mellifera, in Hawaii

作者：Szalanski Allen L^*; Tripodi Amber D; Trammel Clinton E; Downey Danielle

来源：Apidologie, 2016, 47(5): 679-687.

DOI：10.1007/s13592-015-0416-4

• Apis mellifera
• mtDNA
• genetic diversity
• island biogeography

Honey bees, Apis mellifera, in the Hawaiian Islands are geographically isolated from honey bees in mainland United States. We conducted a study on the mitochondrial DNA genetic diversity of honey bees from seven of the Hawaiian Islands by sequencing the intergenic region between the Cytochrome Oxidase I and Cytochrome Oxidase II genes (COI-COII). We observed a total of 10 haplotypes from 235 samples collected from 2009 to 2014. Haplotypes belonged to the A. mellifera C lineage (64 %), M lineage (35 %), and O lineage (1 %). Four of the five C lineage haplotypes found were common among queen breeders in continental United States (C1, C2, C11, C19) and accounted for 99 % of the C lineage bees. Haplotype C33 (1 %) has been observed in feral honey bee populations in continental United States. For the M lineage, which includes the dark honey bee, A. m. mellifera, four haplotypes were observed (M3, M4c"aEuro (TM), M7 and M70), with a novel haplotype unique to Hawaii, M70, being the second most common. Five islands had M lineage haplotypes, with their frequency ranging from 70 % on Maui to 22 % on Molokai. Two individuals of the O lineage, haplotype O1, were found on Oahu. Among the islands, Oahu and Maui, had the greatest amount of haplotypic diversity (haplotypic diversity (H (d) ) = 0.76 and 0.75). Lanai and Kahoolawe had only one haplotype, C1, present.

• 出版日期2016-9