Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation products

作者：Silva Maria Cristina^*; Correa Angelita Duarte; Sousa Pessoa Amorim Maria Teresa; Parpot Pier; Torres Juliana Arriel; Batista Chagas Pricila Maria

来源：Journal of Molecular Catalysis B: Enzymatic , 2012, 77: 9-14.

DOI：10.1016/j.molcatb.2011.12.006

• Environmental biocatalysis
• LC-ESI/MS
• Turnip peroxidase
• Decolorization
• Textile dyes

Peroxidases can be used in decolorization processes and the treatment of textiles effluents. This study evaluates the potential of the turnip peroxidase enzyme in the decolorization of the phthalocyanine textile dye Reactive Blue 21 (RB21). Some factors such as pH, the amount of H2O2 and the enzyme were evaluated in order to determine the optimum conditions for the enzyme performance. The reaction products formed during the decolorization of the RB21 dye were analyzed by high-performance liquid chromatography-mass spectrometry coupling (LC-ESI/MS). LC-ESI/MS analysis showed that the decolorization of the dye RB21 by turnip peroxidase is due to the breaking up of the chromatogenous system. The tests for toxicity towards lettuce seeds showed an increase of the toxicity after enzymatic treatment of the dye. This study verifies the viability of the use of the turnip peroxidase enzyme in the biodegradation of textile dyes.

• 出版日期2012-5