Multi-fed batch culture integrated with three-stage light irradiation and multiple additions of copper ions for the hyperproduction of ganoderic acid and Ganoderma polysaccharides by the medicinal mushroom Ganoderma lucidum

作者：Zhu, Li-Wen; Zhong, Jian-Jiang; Tang, Ya-Jie^*

来源：Process Biochemistry, 2010, 45(12): 1904-1911.

DOI：10.1016/j.procbio.2010.03.010

• Ganoderma lucidum
• Integrated strategy
• Multi-pulse feeding of carbon and nitrogen sources
• Cu2+ multiple additions
• Three-stage light irradiation

To further enhance the accumulation of the bioactive metabolite ganoderic acid (GA) by fermentation of the medicinal mushroom Ganoderma lucidum, a novel integrated strategy was developed by simultaneously adopting a strategy of multiple Cu2+ additions, three-stage light irradiation and multi-pulse feeding of carbon and nitrogen sources. Maximal GA content (i.e., 4.1 mg/100 mg DW) and production (i.e., 720.8 mg/L) were obtained using the novel integrated strategy. Not only the biomass but also the total GA production obtained in this work is the highest reported for a shaker flask culture of G. lucidum. This work is useful for the large-scale production of GA by G. lucidum fermentation.

• 出版日期2010-12
• 单位上海交通大学; 湖北工业大学