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摘要
Objective: The effects of pirfenidone were investigated on cultured human pterygium fibroblasts (HPFs). %26lt;br%26gt;Methods: HPFs were obtained from pterygium surgery and subjected to primary culture. After treatment with 0.5, 1.0 or 1.5mg/mL pirfenidone, MTT and cell migration assays were performed, and procollagen secretion and TGF-beta expression were measured by Western blotting and immunofluorescence analysis. %26lt;br%26gt;Results: Pirfenidone had a significant inhibitory effect on HPF proliferation, migration and collagen synthesis. There were no differences between the cells treated with 0.5, 1.0 and 1.5mg/mL pirfenidone and the controls in the MTT assay. After 48 h of treatment with 1.0 or 1.5mg/mL pirfenidone, TGF-b expression was significantly decreased. %26lt;br%26gt;Conclusions: These findings demonstrate that pirfenidone inhibits the proliferation, migration and procollagen secretion of HPFs at nontoxic concentrations by decreasing TGF-b expression. Thus, pirfenidone may be considered as a safe adjuvant for pterygium surgery to prevent recurrence.
- 出版日期2014-7
