Apatite content of collagen materials dose-dependently increases pre-osteoblastic cell deposition of a cement line-like matrix

作者：Perrier A; Dumas V; Linossier M T; Fournier C; Jurdic P; Rattner A; Vico L; Guignandon A^*

来源：Bone, 2010, 47(1): 23-33.

DOI：10.1016/j.bone.2010.03.010

• Apatite
• Pre-osteoblast
• Osteopontin
• VEGF-A
• Migration

Bone matrix, mainly composed of type I collagen and apatite, is constantly modified during the bone remodeling process, which exposes bone cells to various proportions of mineralized collagen within bone structural units. Collagen-mineralized substrates have been shown to increase osteoblast activities. We hypothesized that such effects may be explained by a rapid secretion of specific growth factors and/or deposition of specific matrix proteins. Using MC3T3-E1 seeded for 32 h on collagen substrates complexed with various apatite contents, we found that pre-osteoblasts in contact with mineralized collagen gave rise to a dose-dependent deposit of Vascular Endothelial Growth Factor-A (VEGF-A) and RGD-containing proteins such as osteopontin (OPN) and fibronectin (FN). This RGD-matrix deposition reinforced the cell adhesion to collagen-mineralized substrates. It was also observed that, on these substrates, this matrix was elaborated concomitantly to an increased cell migration, allowing a homogeneous coverage of the sample. This particular surface activation was probably done firstly to reinforce cell survival (VEGF-A) and adhesion (OPN, FN) and secondly to recruit and prepare surfaces for subsequent bone cell activity.

• 出版日期2010-7