Description of Jeotgalibacillus alkaliphilus sp nov., isolated from a solar salt pan, and Jeotgalibacillus terrae sp nov., a name to replace 'Jeotgalibacillus soli' Chen et al. 2010

作者：Srinivas A; Divyasree B; Sasikala Ch^*; Tushar L; Bharti Dave; Ramana Ch V^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2016, 66(12): 5167-5172.

DOI：10.1099/ijsem.0.001491

A Gram-stain-positive, non-motile, rod-shaped bacterium (strain JC303(T)) isolated from a salt pan was identified based on 16S rRNA gene sequence analysis as a member of the genus Jeotgalibacillus. It was related most closely to Jeotgalibacillus salarius ASL-1(T) (99.1 % similarity), Jeotgalibacillus alimentarius YKJ-13(T) (97.9%), Jeotgalibacillus soli JSM 081008 (97.9%), Jeotgalibacillus malaysiensis D5(T) (97.8 %), Jeotgalibacillus marinus DSM 1297(T) (96.3%), Jeotgalibacillus campisalis SF-57(T) (96.1%) and J. soli P9(T) (94.9%). Genomic relatedness based on DNA-DNA hybridization of strain JC303T with the type strains of the closest related species was less than 40%. Diphosphatidylglycerol, three aminophospholipids, an unidentified aminoglycolipid, two unidentified phospholipids and an unidentified lipid were the polar lipids of strain JC303(T). Major (>10%) fatty acids were anteiso-C-15 : 0, iso-C-15 : 0 and iso-C-14 : 0. Cell-wall amino acids contained peptidoglycan with L-lysine as the diagnostic diamino acid. Strain JC303T contained MK-7 as the predominant (96 %) menaquinone with the presence of a significant amount (4%) of MK-8. The DNA G+C content was 43 mol%. On the basis of morphological, physiological, genotypic, phylogenetic and chemotaxonomic analyses, strain JC303(T) is considered to represent a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus alkaliphilus sp. nov. is proposed. The type strain is JC303(T) (=KCTC 33662(T)=LMG 28756(T)). In addition, we propose to rename J. soli (Chen et al, 2010), an illegitimate homonym of the validly published name Jeotgalibacillus soli (Cunha et al., 2012) as Jeotgalibacillus terrae sp. nov. with type strain JSM 081008(T) (=DSM 22174(T)=KCTC 13528(T)).

• 出版日期2016-12