Antihypertensive effects of Ile-Pro-Ile-Lys from krill (Euphausia superba) protein hydrolysates: purification, identification and in vivo evaluation in spontaneously hypertensive rats

作者：Park Soo Yeon; Je Jae Young; Kang Nalae; Han Eui Jeong; Um Ju Hyung; Jeon You Jin; Ahn Ginnae^*; Ahn Chang Bum^*

来源：European Food Research and Technology, 2017, 243(4): 719-725.

DOI：10.1007/s00217-016-2785-y

• Krill protein hydrolysate
• Angiotensin I-converting enzyme
• Hypertension
• Peptide

Angiotensin I-converting enzyme (ACE) inhibitory peptide was purified from krill (Euphausia superba) protein hydrolysate using consecutive chromatography using an SP-Sephadex C-25 cation exchanger and then reverse-phase high-performance liquid chromatography. The purified ACE inhibitory peptide was identified to be Ile-Pro-Ile-Lys with a molecular weight of 470.3 Da by LC-MS/MS. The IC50 value of Ile-Pro-Ile-Lys was 57.4 mu M. Lineweaver-Burk plots suggest that Ile-Pro-IleLys acts as a non-competitive inhibitor against ACE. Oral administration of Ile-Pro-Ile-Lys significantly (p < 0.05) decreased the systolic blood pressure of spontaneously hypertensive rats. Collectively, an antihypertensive peptide purified from krill protein hydrolysates may be useful as a food ingredient for treatment of hypertension and related diseases.

• 出版日期2017-4