Population Proteomics of the European Hake (Merluccius merluccius)

作者：Gonzalez Elena G; Krey Grigorios; Espineira Montserrat; Diez Amalia; Puyet Antonio; Bautista Jose M^*

来源：Journal of Proteome Research, 2010, 9(12): 6392-6404.

DOI：10.1021/pr100683k

• DIGE technique
• hake
• Merluccius merluccius
• population proteomics
• discriminant analyses
• liver
• brain

We report the novel use of proteomics to investigate protein variation among populations of the European hake (Merluccius merluccius) The liver and brain extracts of 18 hake (N = 36) captured in the Mediterranean Sea, Cantabrian Sea, and Atlantic Ocean were examined by 2D/DIGE and mass spectrometry Significant differences in protein expression among populations were revealed by 84 spots obtained in the gels for the liver and 145 spots for the brain Population groups of samples were defined by multivariate analysis (PCA and hierarchical clustering) According to protein expression levels and the functions of the 55 candidate protein spots identified, which showed significant expression differences, highest population discrimination was rendered by brain proteins involved in cell signaling and metabolism/energy and by liver proteins involved in protein fate Finally, we present a statistically robust framework to accurately classify individuals according to their population of origin Thus purposely identified protein isoforms were found to be competent at discriminating populations These results suggest the possibility of identifying protein biomarkers related to environmental changes in a nonmodel species such as the hake and pave the way for more extensive research on protein variation among populations of marine fishes

• 出版日期2010-12