Mitotic Arrest Induced in Human DU145 Prostate Cancer Cells in Response to KHC-4 Treatment

作者：Shen Cheng Huang; Lin Tien Huang; Hsieh You Liang; Shen Chia Yao; Kuo Sheng Chu; Wu Hsi Chin; Chien Wen Shin; Hsieh Dennis Jine Yuan; Wen Su Ying; Ting Wei Jen; Yao Chun Hsu^*; Huang Chih Yang^*

来源：Environmental Toxicology, 2016, 31(12): 1879-1887.

DOI：10.1002/tox.22189

• KHC-4
• DU145 cells
• antiproliferation
• cell cycle arrest
• cell migration
• inhibition

In this study, the antitumor activity of KHC-4 was analyzed using human prostate cancer (CaP) cells and the underlining anticancer mechanisms of KHC-4 were identified. KHC-4 inhibited cell proliferation and induced cytotoxicity in the castration-resistant CaP DU145 cell line. The most effective concentration of KHC-4 was 0.1 mu M. Cell cycle analysis demonstrated that KHC-4 treatment caused G2/M arrest and a subsequent increase in the sub-G1 population. Furthermore, KHC-4 is up-regulated p21, p27, and p53 in a time-and concentration-dependent manner. The exposure of cells to KHC-4 induced Cdk1/cyclin B1 complex activity, which led to cell cycle arrest. Moreover, KHC-4 inhibited the activities of MMP-2 and MMP-9 to inhibit tumor cell metastasis.

• 出版日期2016-12
• 单位中国医科大学