A 50-Kilodalton Cry2A Peptide Is Lethal to Bombyx mori and Lymantria dispar

作者：Ohsawa Masataka; Tanaka Miki; Moriyama Kenta; Shimazu Mitsuaki; Asano Shin ichiro; Miyamoto Kazuhisa; Haginoya Kohsuke; Mitsui Toshiaki; Kouya Tomoaki; Taniguchi Masayuki; Hori Hidetaka^*

来源：Applied and Environmental Microbiology, 2012, 78(13): 4755-4757.

DOI：10.1128/AEM.07123-11

The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis, and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Va150 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides killed the larvae of both insects.

• 出版日期2012-7