Anti-proliferation effects of ethanolic extracts from Sophora moorcroftiana seeds on human hepatocarcinoma HepG2 cell line

作者：Su, Gang; Yang, Wenke; Meng, Wenbo; Wu, Qionghui; Luo, Yanping; Ma, Xingming; Zhao, Shen; Xie, Xiaodong; Guo, Guang-Qin^*

来源：Natural Product Research, 2018, 32(12): 1472-1475.

DOI：10.1080/14786419.2017.1353503

• Sophora moorcroftiana
• proteomic
• anti-proliferation
• HepG2 cells

Previous studies have shown that the ethanolic extracts from Sophora moorcroftiana seeds (ee-Sms) have in vitro anticancer properties. The anti-proliferation effects of ee-Sms on HepG2 cells were assessed by MTT assay and cell cycle analysis. Total cell proteins were separated by two-dimensional electrophoresis (2-DE), and protein spots with more than two-fold difference were analysed by MALDI-TOF/TOF-MS. MTT assay showed that the anti-proliferation of ee-Sms demonstrates dose- and time dependently. HepG2 cells were treated with ee-Sms at 1.30mg/mL for 48h induced cell cycle arrest in S phase. The differentially-expressed proteins were involved in DNA repair, cell proliferation, cell metabolism and immunoreaction. This study sheds new insights into the molecular mechanisms underlying the anti-proliferation properties of ee-Sms in HepG2 cells.
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• 出版日期2018
• 单位兰州大学