Derivation of human iPSC line NCCSi002-A from umbilical cord blood (UCB) CD34+cells of donor from Indian ethnicity

作者：Fernandes Sophia; Shinde Prajakta; Khan Nikhat; Singh Sanjay; Vardhan Shakti; Nair Velu; Kale Vaijayanti; Limaye Lalita^*

来源：Stem Cell Research, 2018, 26: 80-83.

DOI：10.1016/j.scr.2017.12.006

We discuss the reprogramming of CD34(+) cells isolated from UCB of a healthy female child of Indian ethnicity. The CD34(+) cells were nucleofected using episomal vectors expressing Oct4, Sox2, L-Myc, Klf4, Lin28 and p53DD(negative mutation in p53). The colonies were stained for alkaline phosphatase and evaluated for pluripotency marker expression by PCR, immunofluorescence and flow-cytometry. The safety of cells was confirmed by absence of plasmid in subsequent passages by PCR. G-banded karyotype demonstrated a stable genome. The ability of trilineage differentiation was confirmed by specific marker expression by immunofluorescence invitro and teratoma formation invivo.

• 出版日期2018-1
• 单位中国人民武装警察部队后勤学院