ANALYSIS OF ENZYMATIC DNA-SEQUENCING REACTIONS BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY

作者：SHALER TA; TAN YP; WICKHAM JN; WU KJ; BECKER CH

来源：Rapid Communications in Mass Spectrometry, 1995, 9(10): 942-947.

DOI：10.1002/rcm.1290091015

The products from base-specific, dideoxy-nucleotide chain-termination DNA sequencing reactions catalyzed by the modified T7 DNA polymerase have been analyzed by using the technique of matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry. Preliminary experiments were performed to determine detection limits for a synthetic mixture of mixed-base single-stranded DNA which contained a 14-mer, a 21-mer, and a 41-mer; acceptable spectra, showing peaks for each component, were obtainable for samples that contained as little as 5 fmol per component, Initial sequencing reactions were therefore carried out on 2-pmol amounts of a short synthetic template that was 45 nucleotides in length, employing 2 pmol of 12-mer as the primer strand, This provided readable sequence information out to the 19th base past the primer, Using a 21-mer primer, nearly the entire sequence of the template could be read.

• 出版日期1995