Mutants of with a change in synthesis of N-acyl-homoserine lactones-Signal molecules of quorum sensing regulation

作者：Veselova M A^*; Lipasova V A; Zaitseva Yu V; Koksharova O A; Chernukha M Yu; Romanova Yu M; Khmel I A

来源：Russian Journal of Genetics, 2012, 48(5): 513-521.

DOI：10.1134/S1022795412050213

By means of plasposon mutagenesis, mutants of 370 with the change in production of N-acyl-homoserine lactones (AHL), signal molecules of the Quorum Sensing system of regulation, were obtained. To localize plasposon insertions in mutant strains, fragments of chromosomal DNA containing plasposons were cloned, adjacent DNA regions sequenced, and a search for homologous nucleotide sequences in the GeneBank was initiated. It has been shown that the insertion of plasposon into gene encoding Lon proteinase drastically decreases AHL synthesis. Upon insertion of plasposon into gene encoding phosphoenolpyruvate-synthase, enhancement of AHL production is observed. In mutant carrying inactivated gene , a strong decline of extracellular protease activity, hemolytic, and chitinolytic activities was observed in comparison with the original strain; lipase activity was not changed in this mutant. Mutation in gene did not affect these properties of 370. Mutations in genes and reduced the virulence of bacteria upon infection of mice.

• 出版日期2012-5