A Potentially Polymerizable Heterodinuclear (FeZnII)-Zn-III Purple Acid Phosphatase Mimic. Synthesis, Characterization, and Phosphate Ester Hydrolysis Studies

作者：Zee Yoke Leng Michelle; Gahan Lawrence R^*; Schenk Gerhard

来源：Australian Journal of Chemistry, 2011, 64(3): 258-264.

DOI：10.1071/CH10424

An analogue of the purple acid phosphatase biomimetic 2-((bis(pyridin-2-ylmethyl)amino)methyl)-6-(((2-hydroxybenzyl)(pyridin-2-ylmethyl)amino)methyl)-4-methylphenol has been synthesized. The analogue, 2-((bis(pyridin-2-ylmethyl)amino)methyl)-6-(((2-hydroxy-4-(4-vinylbenzyloxy)benzyl)(pyridin-2-ylmethyl)amino)methyl)-4-methylphenol (H2BPBPMPV) possesses a pendant olefin suitable for copolymerization. Complexation with Fe-III/Zn-II resulted in the complex [(FeZnII)-Zn-III (BPBPMPV)(CH3COO)(2)](ClO4), characterized with mass spectrometry, microanalysis, UV/vis, and IR spectrometry. The catalytic activity of the complex toward bis-(2,4-dinitrophenyl) phosphate was determined, resulting in K-m of 4.1 +/- 0.6 mM, with k(cat) 3.8 +/- 0.2 x 10(-3) s(-1) and a bell-shaped pH-rate profile with pK(a) values of 4.31, 5.66, 8.96, the profile exhibiting residual activity above pH 9.5.

• 出版日期2011