A simple and fast method for quantification of ertapenem using meropenem as internal standard in human plasma in a clinical setting

作者：D'Avolio Antonio^*; Baietto Lorena; De Rosa Francesco Giuseppe; Garazzino Silvia; Sciandra Mauro; Siccardi Marco; Bonora Stefano; Di Perri Giovanni

来源：Therapeutic Drug Monitoring, 2008, 30(1): 90-94.

• ertapenem
• HPLC-UV
• human plasma
• meropenem
• TDM

A high-performance liquid chromatography method for the determination of ertapenem in human plasma using ultraviolet detection (300 nm) was developed and validated. The method involved a simple organic protein precipitation that guarantees rapid sample preparation. By using meropenem as internal standard, it also guarantees improved precision (relative standard deviation <3.70) and accuracy (deviation from true Value <6.58%). The method was linear from 0.59 to 150 mu g/mL and reproducible. In conclusion, this method allows the measurement of ertapenem in human plasma and may be used for both clinical routine applications and pharmacokinetic studies.

• 出版日期2008-2