Embolus-carried vascular endothelial cell growth factor 165 improves angiogenesis in thromboangiitis obliterans

作者：Liu Q; Zhou H C; Yang X L^*; Zhao Y P; Luo H P; He C W; Wan X B

来源：Genetics and Molecular Research, 2014, 13(1): 1744-1752.

DOI：10.4238/2014.March.17.2

• Femoral artery
• Vascular endothelial cell growth factor
• Embolus
• Neovascularization
• Gene therapy

We investigated neovasculization effects of emboluscarried human vascular endothelial cell growth factor 165 (VEGF(165))-encoded adenovirus (Ad) vector in the hindlimbs of rats with thromboangiitis obliterans (TAO). Rats were equally divided into blank control (I), TAO model (II), embolus (III), Ad-VEGF(165) intravascular treatment (IV), Ad-VEGF(165) intramuscular treatment (V), and emboluscarried Ad-VEGF(165) (VI) groups. After interventional treatment, the neovasculization effect of the test gene was observed using immunohistochemistry. At 1 week after administration, compared with group II, groups V and VI had significantly increased microvessel densities, but no significant difference was observed between groups V and VI. At 2 weeks, groups V and VI exhibited significantly increased microvessel densities. At 1 week after administration, compared with group II, both groups V and VI showed a significant difference in the ratio between the alpha- smooth muscle actin count and the muscle fiber count, whereas no significant difference was observed between them. At 2 weeks, groups V and VI also exhibited significant differences in these ratios compared with the other groups. We conclude that Ad-VEGF(165) promotes neovasculization in ischemic limbs. Embolus-carried Ad-VEGF(165) had the most pronounced effect.

• 出版日期2014
• 单位南昌大学