Ultrashort Lipopeptides Self-Assembled with Gold Nanoparticles as Potent Antimicrobial Agents

作者：Fan, Dongxiao; Yao, Chen^*; Zhou, Wenya; Li, Xinsong

来源：Journal of Nanoscience and Nanotechnology, 2018, 18(12): 8124-8132.

DOI：10.1166/jnn.2018.16411

• Antimicrobial Agent
• Lipopeptide
• Nanoparticle
• Self-Assembly
• Biological Toxicity

To develop new antimicrobial synthetic lipopeptides with optimizing peptide length, cationic tripeptides RWR/WRR were N-terminal fatty acylated, and self-assembled with 1-dodecanethiol-anchored gold nanoparticles (Au-DT NPs) via hydrophobic interaction. The ultrashort lipopeptides and their nano-assemblies were effective against a variety of microorganisms, with minimal inhibitory concentrations ranging from 0.5 to 8 mu g/mL. Hemolysis analysis and in vitro cytotoxicity assay revealed that self-assembling with Au-DT NPs would improve biological toxicity of lipopeptides, especially for the most active lipopeptides (Palmitoyl-RWR, Palmitoyl-WRR) with long lipid tails. As lipopeptides/Au-DT NPs displayed slower bactericidal kinetics compared with free lipopeptides, the mode of action was further investigated. Difference in membrane targeting mechanism between lipopeptides and their nano-assemblies may be attributed to the structural architecture. The simple composition and diverse specificities of lipopeptides/Au-DT NPs, as well as their biocompatibility could make them as economically available potent antimicrobial agents for various applications.

• 出版日期2018-12
• 单位东南大学