Non-destructive Phenotypic Analysis of Early Stage Tree Seedling Growth Using an Automated Stereovision Imaging Method

作者：Montagnoli Antonio; Terzaghi Mattia; Fulgaro Nicoletta; Stoew Borys; Wipenmyr Jan; Ilver Dag; Rusu Cristina; Scippa Gabriella S; Chiatante Donato

来源：Frontiers in Plant Science, 2016, 7: 1644.

DOI：10.3389/fpls.2016.01644

• plantphenotype
• biomass
• seedlings
• Picea abies L.
• Pinus sylvestris L.
• Fagus sylvatica L.
• Quercus ilex L.
• RGB image analysis

A plant phenotyping approach was applied to evaluate growth rate of containerized tree seedlings during the precultivation phase following seed germination. A simple and affordable stereo optical system was used to collect stereoscopic redgreenblue (RGB) images of seedlings at regular intervals of time. Comparative analysis of these images by means of a newly developed software enabled us to calculate (a) the increments of seedlings height and (b) the percentage greenness of seedling leaves. Comparison of these parameters with destructive biomass measurements showed that the height traits can be used to estimate seedling growth for needle-leaved plant species whereas the greenness trait can be used for broad-leaved plant species. Despite the need to adjust for plant type, growth stage and light conditions this new, cheap, rapid, and sustainable phenotyping approach can be used to study large-scale phenome variations due to genome variability and interaction with environmental factors.

• 出版日期2016-10-28