Neonatal colonization of mice with LGG promotes intestinal development and decreases susceptibility to colitis in adulthood

作者：Yan, F.^*; Liu, L.; Cao, H.; Moore, D. J.; Washington, M. K.; Wang, B.; Peek, R. M.; Acra, S. A.; Polk, D. B.^*

来源：Mucosal Immunology, 2017, 10(1): 117-127.

DOI：10.1038/mi.2016.43

• LACTOBACILLUS-RHAMNOSUS GG
• PLACEBO-CONTROLLED TRIAL
• GROWTH-FACTOR RECEPTOR
• HUMAN GUT MICROBIOME
• EPITHELIAL-CELLS
• BACTERIAL DIVERSITY
• DEPENDENT MECHANISM
• SOLUBLE-PROTEIN
• ATOPIC DISEASE
• BREAST-MILK

Development of the intestinal microbiota during early life serves as a key regulatory stage in establishing the hostmicrobial relationship. This symbiotic relationship contributes to developing host immunity and maintaining health throughout the life span. This study was to develop an approach to colonize conventionally raised mice with a model probiotic bacterium, Lactobacillus rhamnosus GG (LGG), and to determine the effects of LGG colonization on intestinal development and prevention of colitis in adulthood. LGG colonization in conventionally raised was established by administering LGG to pregnant mice starting at gestational day 18 and pups at postnatal days 1-5. LGG colonization promoted bodyweight gain and increased diversity and richness of the colonic mucosa-associated microbiota before weaning. Intestinal epithelial cell proliferation, differentiation, tight junction formation, and mucosal IgA production were all significantly enhanced in LGG-colonized mice. Adult mice colonized with LGG showed increased IgA production and decreased susceptibility to intestinal injury and inflammation induced in the dextran sodium sulfate model of colitis. Thus, neonatal colonization of mice with LGG enhances intestinal functional maturation and IgA production and confers lifelong health consequences on protection from intestinal injury and inflammation. This strategy might be applied for benefiting health in the host.

• 出版日期2017-1
• 单位天津医科大学