A novel triple adjuvant formulation promotes strong, Th1-biased immune responses and significant antigen retention at the site of injection

作者：Wilson Heather L; Kovacs Nolan Jennifer; Latimer Laura; Buchanan Rachelle; Gomis Susantha; Babiuk Lorne; Littel van den Hurk Sylvia van Drunen^*

来源：Vaccine, 2010, 28(52): 8288-8299.

DOI：10.1016/j.vaccine.2010.10.006

• Adjuvants
• Fluorescent imaging
• Polyphosphazenes
• Antigen retention

Ovalbumin (OVA) was labeled with a near infra-red dye (*OVA) and formulated with the host defense peptide indolicidin (Indol), CpG oligodeoxynucleotide (ODN) 1826 (CpG) and/or poly(p-dicarboxylatophenoxy)-phosphazene (PP4). The immunogenicity of these *OVA formulations was evaluated in mice. All double and triple adjuvant combinations elicited strong antibody responses. *OVA formulated with CpG ODN in combination with indolicidin, PP4 or both induced only IFN-gamma, while formulations with indolicidin and/or PP4 promoted predominantly IL-5 production. Overall, both IgG and IFN-gamma production was superior when *OVA was combined with CpG/Indol/PP4. Furthermore, mice injected with *OVA formulated with CpG/Indol/PP4 contained detectable *OVA in the injection site two months post immunization. These results indicate that the CpG/Indol/PP4 combination promotes prolonged antigen retention and strong, antigen-specific Th1-biased immune responses.

• 出版日期2010-12-6