alpha 1,3 Fucosyltransferase VII plays a role in colorectal carcinoma metastases by promoting the carbohydration of glycoprotein CD24

作者：Li Wenhua; Zhang Wen^*; Luo Jianming; Cao Ayong; Zhang Ying; Huang Dan; Sheng Weiqi; Cai Sanjun; Li Jin

来源：Oncology Reports, 2010, 23(6): 1609-1617.

DOI：10.3892/or_00000802

• alpha l,3 Fucosyltransferase VII
• colorectal carcinoma
• metastasis
• CD24
• slatyl Lewis X

Changes in the expression levels of alpha 1,3 FucT-VII and carbohydrate product SLe(x) have been reported in certain types of malignant transformations. However, the specific role of FucT-VII in human colorectal carcinoma is still not clear. We evaluated the expression of FucT-VII in tumor specimens from 30 colorectal carcinoma patients by RT-PCR and immunohistochemistry. The alteration of metastatic potential of LOVO cells before and after alpha 1,3 FucT-VII gene transfection was also assayed. The expression change of glycoprotein CD24 and carbohydrate antigen SLex after stable transfection of LOVO was also examined in vitro. Higher mRNA and protein expression of FucT-VII were observed in colorectal tumors compared with adjacent normal ones. The cell chemotactic migration and invasion were enhanced after alpha 1,3 FucT-VII transfection in LOVO cells. There was a positive correlation between alpha 1,3 FucT-VII mRNA expression and lymph node status (p=0.009) and AJCC stage (p=0.007), similar correlation was also observed at protein level (p=0.042 and 0.022, respectively). Overexpression of alpha 1,3 FucT-VII promoted the expression of CD24 and SLe(x) in LOVO cells. Our findings suggest that alpha 1,3 FucT-VII might play a role in colorectal carcinoma metastases by promoting the carbohydration of glycoprotein CD24.

• 出版日期2010-6
• 单位复旦大学