Antitumor Effect of Combination of Hyperthermotherapy and 5-Aminolevulinic acid (ALA)

作者：Takahashi Kiwamu^*; Hasegawa Takeo; Ishii Takuya; Suzuki Atsuko; Nakajima Motowo; Uno Kazuko; Yasuda Iuko; Kishi Atsuko; Sadamoto Kaori; Abe Fuminori; Tanaka Tohru

来源：Anticancer Research, 2013, 33(7): 2861-2866.

• 5-Aminolevulinic acid
• ALA
• protoporphyrin IX
• hyperthermia
• animal tumor model

Background: 5-Aminolevulinic acid (ALA) is a precursor of heme. ALA is used as a photosensitive substance in photodynamic diagnosis (PDD) and photodynamic therapy (PDT) because heme metabolism is abnormal in tumor cells and a photosensitive metabolite of heme synthesis from ALA, protoporphyrin IX (PpIX), specifically accumulates in tumors. We investigated the enhancement of the antitumor effect by combination of ALA and hyperthermotherapy (HT) using a transplanted tumor model with Lewis lung carcinoma cells (3LL) in mice. Materials and Methods: Animals were divided into four test groups: control (untreated), HT, and HT plus ALA (100 or 300 mg/kg) groups, and HT by bathing at 43 degrees C for 20 mm was performed at five days after transplantation. ALA was administered once at the above doses three hours before HT by intraperitoneal injection. Results: The tumor sizes at five days after HT were 5.2- and 2.6-times greater than those at the tune of HT in the control and HT groups, respectively. In contrast, PpIX accumulation in the tumor region was noted three hours after ALA administration, the HT+ALA group given at 100 or 300 mg/kg of ALA inhibited tumor growth to 1.3- and 1.1-times increases in the tumor size. Conclusion: Therefore, ALA administration markedly enhanced the tumor growth-inhibitory effect of HT.

• 出版日期2013-7