Anti-inflammatory and antinociceptive action of the dimeric enkephalin peptide biphalin in the mouse model of colitis: New potential treatment of abdominal pain associated with inflammatory bowel diseases

作者：Sobczak Marta; Pilarczyk Andrzej; Jonakowski Mateusz; Jarmuz Agata; Salaga Maciej; Lipkowski Andrzej W; Fichna Jakub^*

来源：Peptides, 2014, 60: 102-106.

DOI：10.1016/j.peptides.2014.08.005

• Biphalin
• Inflammatory bowel diseases
• Inflammatory pain
• Mixed opioid agonist
• Opioid receptors

Biphalin a mixed MOP/DOP agonist, displays a potent antinociceptive activity in numerous animal models of pain. The aim of the study was to characterize the anti-inflammatory and antinociceptive action of biphalin in the mouse models of colitis. The anti-inflammatory effect of biphalin (5 mg/kg, twice daily, i.c. and i.p.) was characterized in a semi-chronic mouse model of colitis, induced by i.c. injection of trinitrobenzenesulfonic acid (TNBS). The antinociceptive action of biphalin (5 mg/kg, i.p. and i.c.) in inflamed mice was assessed in mustard oil-induced model of visceral pain and in the hot plate test. In the semi-chronic mouse model of colitis, biphalin i.c. (5 mg/kg), but not i.p. improved colitis macroscopic score (2.88 +/- 0.19 and 4.99 +/- 0.80 units for biphalin and vehicle treated animals, respectively). Biphalin injected i.p. and i.c. (5 mg/kg) displayed a potent antinociceptive action in the mustard oil-induced pain test. In the hot plate test, biphalin (5 mg/kg, i.p.) produced a potent antinociceptive activity in inflamed mice, suggesting central site of action. Our data suggest that biphalin may become a novel opioid-based analgesic agent in IBD therapy and warrant further investigation of its pharmacological profile.

• 出版日期2014-10