Analysis of a Jup hypomorphic allele reveals a critical threshold for postnatal viability

作者：Swope David; Li Jifen; Muller Eliane J; Radice Glenn L^*

来源：Genesis, 2012, 50(10): 717-727.

DOI：10.1002/dvg.22034

• cell adhesion
• intercalated disc
• desmosome
• adherens junction
• cadherin
• heart

Mutations in the human Jup gene cause arrhythmogenic right ventricular cardiomyopathy (ARVC), a heart muscle disease that often leads to sudden cardiac death. Inactivation of the murine Jup gene (also known as plakoglobin) results in embryonic lethality due to cardiac rupture. In an effort to generate a conditional knockout allele, a neomycin cassette was introduced into the murine plakoglobin (PG) gene. This allele (PG FN) functions as a hypomorph when combined with a null allele (PG ?). About half of the PG FN/? animals were smaller than their littermates and died before weaning age, whereas the remaining PG FN/? animals survived. Despite the reduced levels of PG in the heart, there were no signs of cardiomyopathy or cardiac dysfunction as determined by echocardiography. Importantly, the PG homolog, beta-catenin (CTNNB1), was increased in the PG FN/? hearts. In addition to its structural role as part of the N-cadherin/catenin adhesion complex, beta-catenin is a downstream effector of Wnt signaling. However, no change in beta-catenin/TCF reporter activity was observed in PG FN/? embryos suggesting that excess beta-catenin was not likely causing increased transcription of Wnt/beta-catenin target genes. These data suggest novel s) for PG beyond the heart and define a critical threshold of PG expression that is necessary for postnatal survival. genesis 50:717727, 2012.

• 出版日期2012-10