A comprehensive analysis of the COL29A1 gene does not support a role in eczema

作者：Naumann Aline; Soderhall Cilla; Foelster Holst Regina; Baurecht Hansjoerg; Harde Viola; Mueller Wehling Konstanze; Rodriguez Elke; Ruether Andreas; Franke Andre; Wagenpfeil Stefan; Novak Natalija; Mempel Martin; Kalali Behnam Naderi; Allgaeuer Michael; Koch Jeanette; Gerhard Markus; Melen Erik; Wahlgren Carl Fredrik; Kull Inger; Stahl Caroline; Pershagen Goran; Lauener Roger; Riedler Josef; Doekes Gert; Scheynius Annika; Illig Thomas; von Mutius Erika

来源：Journal of Allergy and Clinical Immunology, 2011, 127(5): 1187-U471.

DOI：10.1016/j.jaci.2010.12.1123

• Atopic dermatitis
• eczema
• genetics
• Col29A1

Background: Based on a recent positional cloning approach, it was claimed that the collagen 29A1 gene (COL29A1), which encodes an epidermal collagen, represents a major risk gene for eczema underlying a previously reported linkage to chromosome 3q21. However, thus far, not a single replication attempt has been published, and no definitive functional data have been provided.
Objectives: We aimed to determine whether COL29A1 polymorphisms contribute to eczema susceptibility and whether COL29A1 expression is altered in eczema.
Methods: We investigated the reported association of COL29A1 variants with eczema, subtypes of eczema, and eczema-related traits in 5 independent and large study populations comprehensively phenotyped for allergic diseases: a set of 1687 German patients with eczema and 2387 population control subjects, a collection of 274 German families with eczema-diseases children, a cross-sectional population of German children (n = 3099), the Swedish population-based birth cohort Children Allergy and Milieu in Stockholm, an Epidemiologic Study (BAMSE) (n=2033), and the European cross-sectional Prevention of Allergy-Risk Factors for Sensitization Related to Farming and Anthroposophic Lifestyle (PARSIFAL) study (n = 3113). An additional set of 19 COL29A1 coding single nucleotide polymorphisms was analyzed in BAMSE and PARSIFAL. COL29A1 expression was investigated by using in situ hybridization.
Results: We found no evidence for a relationship between COL29A1 polymorphisms and eczema. The equivalence test rejected the hypothesis of association even excluding small effects. In situ hybridization carried out on biopsy specimens from lesional and nonlesional skin of patients with eczema and from healthy control subjects did not show any differences in the cellular distribution pattern of COL29A1 expression at the mRNA level.
Conclusions: Our results suggest that COL29A1 is unlikely to contain genetic variants that have a major effect on eczema or atopy susceptibility. (J Allergy Clin Immunol 2011;127:1187-94.)

• 出版日期2011-5
• 单位河北医科大学