科研之友
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摘要
Synthetic polymeric systems are considered vitally important in tissue regeneration. The reasons lay behind their specific characteristics such as porosity, hydrophilicity, degradation time, and mechanical properties which are variable to a large extent. The segmented polyurethanes have been used as biomaterials in medical field because they possess unique properties. In this study, the perforated polyurethane (PPU) scaffold as cartilage substitution was prepared and evaluated through biological assays. During the in vitro stage, the behaviour of both primary chondrocyte cells and L929 cell line in presence of PPU were examined. To evaluate in vivo performance of the scaffolds, PPUs were implanted in the scapula cartilage of twelve mixed-bred female sheep and comparisons were made with the implanted autologous cartilages as control. The results of in vitro studies showed some round and aggregated chondrocyte cells grown after 2 weeks of cell culture along with a considerable growth and attachment of L929 cells. In vivo studies showed that there is no significant difference between the restored PPUs and the autologous cartilage control. Our studies support the potential use of PPU as a proper substitute or rather as an assistant in cartilage repair. Obviously, further studies are needed to qualify the constituted cartilages regarding proteoglycan and collagen productions.
- 出版日期2010-6
