Establishment of CD5 and CD10 double-positive mature B-cell line, WILL1, showing complex 8q24 translocation involving 14q32 and 6q27

作者：Uneda Shima; Gotoh Minako; Sonoki Takashi^*; Nishida Kazuhiro; Nakamura Yasushi; Kurimoto Miwa; Hanaoka Nobuyoshi; Matsuoka Hiroshi; Taniwaki Masafumi; Nakakuma Hideki

来源：International Journal of Hematology, 2008, 88(5): 536-542.

DOI：10.1007/s12185-008-0189-x

• Lymphoma
• CD5
• CD10
• Translocation
• 8q24
• 6q27

We established a novel mature B-cell line from a CD5 and CD10 double-positive diffuse large B-cell lymphoma patient, designated as WILL1. WILL1 cells were positive for CD5, CD10, CD19, and CD20. Spectral karyotype (SKY) analysis revealed chromosome 8 signals on 6q27 as well as 14q32. Fluorescence in situ hybridization (FISH) analysis suggested that a translocation break occurred outside the immunoglobulin heavy chain (IGH) gene on 14q32. Moreover, fusion signals of IGH and C-MYC probes were detected on the derivative 6 and derivative 14 chromosomes. Southern blot analysis using a C-MYC exon II fragment failed to detect rearrangement, suggesting that the 8q24 breakpoints lay far up- or downstream of the C-MYC gene. WILL1 is a useful tool to analyze the pathogenesis of CD5 and CD10 double-positive diffuse large B-cell lymphoma, and for molecular cloning of the unique translocation breakpoints of 14q32 and 8q24 and a novel gene on 6q27.

• 出版日期2008-12