An Amperometric D-Amino Acid Biosensor Prepared with a Thermostable D-Proline Dehydrogenase and a Carbon Nanotube-Ionic Liquid Gel

作者：Tani Yuji; Itoyama Yukiko; Nishi Kenichi; Wada Chikahiro; Shoda Yoshio; Satomura Takenori; Sakuraba Haruhiko; Ohshima Toshihisa; Hayashi Yukako; Yabutani Tomoki^*; Motonaka Junko

来源：Analytical Sciences, 2009, 25(7): 919-923.

DOI：10.2116/analsci.25.919

Carbon nanotube (CNT)gel, which is composed of a mixture of single-wall CNT, ail ionic liquid, thermostable D-proline dehydrogenase (D-Pro DH) immobilized electrode was utilized for the determination of D-amino acids (DAAs) in food samples. When a critical comparison with CNT, Ketjen Black (KB), and carbon powder (CP) was also carried Out, the CNT/D-Pro DH immobilized electrode showed the highest sensitivity and the lowest detection limit of D-proline. In addition, the CNT/D-Pro DH immobilized electrode was applied to detection of DAAs in rice wine and vinegar samples. The concentrations of DAAs in rice wine and vinegar samples were 0.0210 /- 0.0001 and 0.55 /- 0.05 mmol L(-1). respectively.

• 出版日期2009-7