Facile measurement of H-1-N-15 residual dipolar couplings in larger perdeuterated proteins

作者：Fitzkee Nicholas C; Bax Ad^*

来源：Journal of Biomolecular NMR, 2010, 48(2): 65-70.

DOI：10.1007/s10858-010-9441-9

• ARTSY
• Catalytic core domain
• HIV integrase
• Quantitative J correlation
• TROSY
• RDC

We present a simple method, ARTSY, for extracting (1)J(NH) couplings and H-1-N-15 RDCs from an interleaved set of two-dimensional H-1-N-15 TROSY-HSQC spectra, based on the principle of quantitative J correlation. The primary advantage of the ARTSY method over other methods is the ability to measure couplings without scaling peak positions or altering the narrow line widths characteristic of TROSY spectra. Accuracy of the method is demonstrated for the model system GB3. Application to the catalytic core domain of HIV integrase, a 36 kDa homodimer with unfavorable spectral characteristics, demonstrates its practical utility. Precision of the RDC measurement is limited by the signal-to-noise ratio, S/N, achievable in the 2D TROSY-HSQC spectrum, and is approximately given by 30/(S/N) Hz.

• 出版日期2010-10