ADSORPTION OF NUCLEASE P1 ON CHITOSAN NANO-PARTICLES

作者：Shi Lu E^*; Tang Zhen Xing

来源：Brazilian Journal of Chemical Engineering, 2009, 26(2): 435-443.

DOI：10.1590/s0104-66322009000200022

• Chitosan
• Nano-particles
• Sorption kinetics
• Sorption mechanism

The sorption of nuclease P1 onto chitosan nano-particles is studied in this paper. The effect of some adsorption kinetics factors such as nuclease P1 concentration, chitosan nano-particles solution concentration, adsorption temperature, chitosan nano-particles size, solution pII, etc. is investigated. Adsorption of nuclease P1 onto chitosan nano-particles is fitted into Lagergren first-order equation at initial nuclease P1 concentration of 3.0 mg/mL. The first-order constant for nuclease P1 is 22.98 h(-1). When nuclease P1 concentration is controlled into certain region, the adsorption fits into Freundlich isothermal linear equation. A mechanism of adsorption for nuclease P1 is proposed by analyzing IR spectra. The IR spectra shows that the hydrogen bond might be the main force between the hydroxyl group, the NH(2) group and the nuclease P1.

• 出版日期2009-6
• 单位杭州师范大学