Assessing Bacillus subtilis biosurfactant effects on the biodegradation of petroleum products

作者：Montagnolli Renato Nallin; Matos Lopes Paulo Renato; Bidoia Ederio Dino^*

来源：Environmental Monitoring and Assessment, 2015, 187(1): 4116.

DOI：10.1007/s10661-014-4116-8

• Bioremediation
• Respirometry
• Kinetics
• Hydrocarbon

Microbial pollutant removal capabilities can be determined and exploited to accomplish bioremediation of hydrocarbon-polluted environments. Thus, increasing knowledge on environmental behavior of different petroleum products can lead to better bioremediation strategies. Biodegradation can be enhanced by adding biosurfactants to hydrocarbon-degrading microorganism consortia. This work aimed to improve petroleum products biodegradation by using a biosurfactant produced by Bacillus subtilis. The produced biosurfactant was added to biodegradation assays containing crude oil, diesel, and kerosene. Biodegradation was monitored by a respirometric technique capable of evaluating CO2 production in an aerobic simulated wastewater environment. The biosurfactant yielded optimal surface tension reduction (30.9 mN m(-1)) and emulsification results (46.90 % with kerosene). Biodegradation successfully occurred and different profiles were observed for each substance. Precise mathematical modeling of biosurfactant effects on petroleum degradation profile was designed, hence allowing long-term kinetics prediction. Assays containing biosurfactant yielded a higher overall CO2 output. Higher emulsification and an enhanced CO2 production dataset on assays containing biosurfactants was observed, especially in crude oil and kerosene.

• 出版日期2015-1
• 单位Univ Estadual Paulista