Agromyces binzhouensis sp nov., an actinobacterium isolated from a coastal wetland of the Yellow River Delta

作者：Chen, Zhirong; Guan, Yingying; Wang, Jingjing; Li, Jing^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2016, 66(6): 2278-2283.

DOI：10.1099/ijsem.0.001022

• DEOXYRIBONUCLEIC-ACID
• EMENDED DESCRIPTION
• SUBSP HIPPURATUS
• GEN. NOV.
• RECLASSIFICATION
• EXTRACTION
• BACTERIA
• FUCOSUS
• MEMBER
• SOIL

A Gram-stain-positive, heterotrophic, non-spore-forming, rod-shaped strain, designated OAct353(T), belonging to the genus Agromyces was isolated from a soil sample collected from a coastal wetland of the Yellow River delta, PR China. The strain was identified using a polyphasic taxonomic approach. The strain grew in the presence of 0.10% (w/v) NaCl (optimum 2-3 %), at pH 5.0-8.0 (optimum pH 7.0) and 12-36 degrees C (optimum 28 degrees C). The isolate contained 2,4-diaminobutyric acid, glutamic acid and glycine in its peptidoglycan. The acyl type of the cell-wall muramic acid was N-acetyl. The whole-cell sugars of this novel strain were glucose, xylose and rhamnose. The predominant menaquinones were MK-12 (74%) and MK-11 (21 %). The major phospholipids were phosphatidylglycerol, one unknown phospholipid, three unknown glycolipids and three unknown polar lipids. The major fatty acids were iso-C-16:0, anteiso-C-15:0 and anteiso-C-17:0. The DNA G+C content was 69.6 mol %. DNA-DNA relatedness clearly separated strain OAct353(T) from its closest relatives. On the basis of phenotypic, phylogenetic and chemotaxonomic data, a novel species, Agromyces binzhouensis sp. nov., is proposed. The type strain is OAct353(T) (=CGMCC4.7180(T)=DSM 28305(T)=NRRL B-59115(T)).

• 出版日期2016-6
• 单位中国海洋大学