Discovery of Benzo[f]indole-4,9-dione Derivatives as New Types of Anti-Inflammatory Agents

作者：Chen You Ren; Tseng Chih Hua; Chen Yeh Long; Hwang Tsong Long^*; Tzeng Cherng Chyi

来源：International Journal of Molecular Sciences, 2015, 16(3): 6532-6544.

DOI：10.3390/ijms16036532

Certain benzo[f]indole-4,9-dione derivatives were synthesized and evaluated for their inhibitory effects on superoxide anion generation and neutrophil elastase (NE) release in formyl-l-methionyl-l-leucyl-l-phenylalanine (fMLF)-activated human neutrophils. Results indicated that (Z)-1-benzyl-4-(hydroxyimino)-1H-benzo[f]indol-9(4H)-one (10) showed a potent dual inhibitory effect on NE release and superoxide anion generation with IC50 value of 2.78 and 2.74 mu M respectively. The action mechanisms of 10 in human neutrophils were further investigated. Our results showed that compound 10 did not alter fMLF-induced phosphorylation of Src (Src family Y416). Notably, phosphorylation of Akt (S473) and mobilization of [Ca2+](i) caused by fMLF was inhibited by compound 10. Further structural optimization of 10 is ongoing.

• 出版日期2015-3
• 单位长春大学