Albumin-Assisted Method Allows Assessment of Release of Hydrophobic Drugs From Nanocarriers

作者：Gil Dmitry; Frank Kamenetskii Anastasia; Barry John; Reukov Vladimir; Xiang Yun; Das Arabinda; Varma Abhay K; Kindy Mark S; Banik Naren L; Vertegel Alexey^*

来源：Biotechnology Journal, 2018, 13(1): 1700337.

DOI：10.1002/biot.201700337

• albumin
• drug release
• liposomes
• membrane dialysis
• nanoparticles

Polymeric nanoparticles have been extensively studied as drug delivery vehicles both in vitro and in vivo for the last two decades. In vitro methods to assess drug release profiles usually utilize degradation of nanoparticles in aqueous medium, followed by the measurement of the concentration of the released drug. This method, however, is difficult to use for drugs that are poorly water soluble. In this study, a protocol for measuring drug release kinetic using albumin solution as the medium is described. Albumin is a major blood transport protein, which mediates transport of many lipid soluble compounds including fatty acids, hormones, and bilirubin. The use of a dialysis-based system utilizing albumin dialysate solution allows hydrophobic drug release from a diverse set of drug delivery modalities is demonstrated. The method using liposomes and PLGA nanoparticles as drug carriers, and two model hydrophobic drugs, 17-estradiol, and dexamethasone is validated.

• 出版日期2018-1