Use of silver nanoparticles for managing Gibberella fujikuroi on rice seedlings

作者：Jo Young Ki^*; Cromwell William; Jeong Hae Kwon; Thorkelson John; Roh Jae Hwan; Shin Dong Bum

来源：Crop Protection, 2015, 74: 65-69.

DOI：10.1016/j.cropro.2015.04.003

• Gibberella fujikuroi
• Rice
• Seedborne diseases
• Seed treatment
• Silver nanoparticle

It is of great interest for rice producers to develop novel seed disinfection methods with effective, less toxic, and environment-friendly active ingredients. This study evaluated silver nanoparticles (AgNP) as an alternative agent for seed treatment since elemental silver possesses strong antimicrobial properties and a low toxicity to humans and animals. AgNP (similar to 7.5 nm in diameter) solution was chemically synthesized and evaluated for antifungal activity against Gibberella fujikuroi as representing a seedborne fungal pathogen in rice (Oryza sativa). AgNP reduced viability of G. fujikuroi conidia by 50% when directly exposed to concentrations ranging from 0.015 to 1.5 mu g mL(-1) for 1 to 20 min. Rice seeds infested with G. fujikuroi and treated with 150 mu g mL(-1) of AgNP significantly decreased colony-forming units (CFU) of G. fujikuroi on the seed surface when treated for 10 min and up to 24 h. Seed treatment with 150 mu g mL(-1) AgNP for 12 or 24 h significantly improved seedling emergence and height of G. fujikuroi-infested seeds. Adverse effects on germination rate and seedling growth were not observed with any of the AgNP treatments (<150 mu g mL(-1) for up to 48-h exposure) tested in this study. The antifungal effect of AgNP against G. fujikuroi attested in this study suggests that AgNP is a new antifungal ingredient that can be used for managing important seedborne fungal pathogens of rice.

• 出版日期2015-8