Degradation characteristics and applications of Amycolatopsis sp. M3-1 for ZJ0273

作者：Cai, Zhi-Qiang; Xu, Xiang-Hong; Chen, Qiao-Li; Zhao, Xi-Yue; Wang, Hai-Yan; Huang, Lei; Ye, Qing-Fu^*

来源：Journal of Chemical Engineering of Chinese Universities, 2012, 26(1): 93-98.

• Amycolatopsis sp. M3-1
• Biotechnological applications
• Degradation characteristics
• Degrading bacteria
• Degrading character
• Microbiological methods
• Molecular-biological techniques
• ZJ0273, propyl 4-(2-(4, 6-dimethoxypyrimidin-2-yloxy) benzylamino) benzoate

ZJ0273, Propyl 4-(2-(4, 6-dimethoxypyrimidin-2-yloxy) benzylamino) benzoate, is a novel herbicide with the property of high activity, broad spectrum and low toxicity to animals, and it is developed for pre- and post-emergence weed control in the oilseed rape field. The highly efficient ZJ0273 degrading bacterial strain M3-1 was screened, isolated and separated from farmland of long-term using pyrimidinylthiobenzoate herbicides by applying modern molecular biological techniques together with the traditional microbiological methods, and it can grow on by using ZJ0273 as the sole carbon and energy source. The strain M3-1 was identified as Amycolatopsis sp. by physiological, biochemical tests and 16s rDNA sequence. The optimum ZJ0273 degrading conditions of using strain M3-1 and their application in soil were studied. The results show that with condition of 35°C, pH 6.0, 8% of inoculation and 100 mg·L-1of ZJ0273, the degrading ratio in 25 d is 63.3%. Using strain M3-1, the degradation ratio of ZJ0273 is decreased with the increasing ZJ0273 concentration;300 mg·L-1of ZJ0273 can strongly inhibit bio-activity of strain M3-1, which means that higher concentration of ZJ0273 has higher toxicity to M3-1. The strain M3-1also has a good degradation effect to ZJ0273 in soil, the degradation ratio could up to 53.7% after 25 days. The physicochemical properties of strain M3-1 may open new possibilities for its biotechnological applications and allow the use of Amycolatopsis sp. M3-1 in the soil bioremediation.

• 出版日期2012-2
• 单位浙江大学