A LINKAGE MAP OF MOUSE CHROMOSOME-1 USING AN INTERSPECIFIC CROSS SEGREGATING FOR THE GLD AUTOIMMUNITY MUTATION

作者：WATSON ML; DEUSTACHIO P; MOCK BA; STEINBERG AD; MORSE HC; OAKEY RJ; HOWARD TA; ROCHELLE JM; SELDIN MF

来源：Mammalian Genome, 1992, 2(3): 158-171.

DOI：10.1007/BF00302874

An interspecific backcross was used to define a high resolution linkage map of mouse Chromosome (Chr) 1 and to analyze the segregation of the generalized lymphoproliferative disease (gld) mutation. Mice homozygous for gld have multiple features of autoimmune disease. Analysis of up to 428 progeny from the backcross [(C3H/HeJ-gld X Mus spretus)F1 X C3H/HeJ-gld] established a map that spans 77.6 cM and includes 56 markers distributed over 34 ordered genetic loci. The gld mutation was mapped to a less than 1 cM segment on distal mouse Chr 1 using 357 gld phenotype-positive backcross mice. A second backcross, between the laboratory strains C57BL/6J and SWR/J, was examined to compare recombination frequency between selected markers on mouse Chr 1. Significant differences in crossover frequency were demonstrated between the interspecific backcross and the inbred laboratory cross for the entire interval studied. Sex difference in meiotic crossover frequency was also significant in the laboratory mouse cross. Two linkage groups known to be conserved between segments of mouse Chr 1 and the long arm of human Chrs 1 and 2 where fur-ther defined and a new conserved linkage group was identified that includes markers of distal mouse Chr 1 and human Chr 1, bands q32 to q42.

• 出版日期1992