Molecular identification of Mycobacterium bovis and the importance of zoonotic tuberculosis in Mexican patients

作者：Portillo Gomez L^*; Sosa Iglesias E G

来源：International Journal of Tuberculosis and Lung Disease, 2011, 15(10): 1409-1414.

DOI：10.5588/ijtld.10.0608

• zoonotic tuberculosis
• M. bovis
• PCR
• epidemiology

SETTING: The epidemiology of zoonotic tuberculosis (ZTB) in humans in Mexico is poorly known.
OBJECTIVE: To identify isolates of Mycobacterium bovis in humans and cattle by polymerase chain reaction (PCR), and establish the clinical and epidemiological importance of ZTB in humans.
DESIGN: From 1995 to 2009, 124 isolates from patients with TB and 60 isolates from cattle were analysed. PCR identification was performed using the oxyR gene, and the clinical and epidemiological aspects of ZTB in humans were investigated.
RESULTS: PCR identified 93 M. bovis isolates: 35 (28%) from the 124 human isolates and 58 (97%) from the 60 cattle isolates. The sensitivity and specificity of the method were 100%. ZTB in the 35 patients presented as extra-pulmonary TB (EPTB) in 74%: 51% were children, 69% had malnutrition, 51% had consumed unpasteurised milk and 6% had contact with animals; 11% were relapses and 31% died.
CONCLUSIONS: PCR using the oxyR gene is highly sensitive, specific and rapid for the identification of M. bovis. ZTB is a serious public health problem, and presented as EPTB in children with malnutrition and those who had consumed unpasteurised milk. ZTB provokes relapses and a high mortality rate.

• 出版日期2011-10