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摘要
A potent alpha-glucosidase inhibitor (compound I) was isolated from coffee brews by activity-based fractionation and identified as a beta-carboline alkaloid norharman (9H-pyrido[3.4-b]indole) on the basis of mass spectroscopy and nuclear magnetic resonance spectra (H-1 NMR, C-13 NMR, and COSY). The norharman showed potent inhibition against alpha-glucosidase enzyme in a concentration-dependent manner, with an IC50 value of 0.27 mM for maltase and 0.41 mM for sucrase. A Lineweaver-Burk plot revealed that norharman inhibited alpha-glucosidase enzyme uncompetitively, with a K-i value of 0.13 mM.
- 出版日期2015-2
