A chemically modified lipase preparation for catalyzing the transesterification reaction in even highly polar organic solvents

作者：Solanki Kusum; Gupta Munishwar Nath^*

来源：Bioorganic & Medicinal Chemistry Letters, 2011, 21(10): 2934-2936.

DOI：10.1016/j.bmcl.2011.03.059

• Chemical modification
• Pyromellitic dianhydride
• Pseudomonas cepacia lipase
• Low water organic media
• Transesterification reactions
• Crosslinked protein coated microcrystals

Acylation of Pseudomonas cepacia lipase with Pyromellitic dianhydride to modify 72% of total amino groups was carried out. Different organic solvents were screened for precipitation of modified lipase. It was found that 1,2-dimethoxyethane was the best precipitant which precipitated 97% protein and complete activity. PCMC (protein coated microcrystals), CLPCMC (crosslinked protein coated microcrystals), EPROS (enzyme precipitated and rinsed with organic solvents) and pH tuned preparations of modified and unmodified lipase were prepared and used for carrying out transesterification reaction with n-octane and dimethyl formamide (DMF) as reaction medium. In n-octane, among all the preparations, CLPCMC of modified lipase gave highest rate (1970 nmol min (1) mg (1)) as compared to unmodified pH tuned lipase (128 nmol min (1) mg (1)). In DMF, with both 1% (v/v) and 5% (v/v) water content, CLPCMC showed highest initial rate of 0.72 and 7.2 nmol min (1) mg (1), respectively. Unmodified pH tuned lipase showed no activity at all in DMF with both 1% and 5% (v/v) water content.

• 出版日期2011-5-15