functional chitosan membrane with grafted epigallocatechin-3-gallate and lovastatin enhances periodontal tissue regeneration in dogs

作者：Lee Bor Shiunn; Lee Chien Chen; Lin Hung Pin; Shih Wei An; Hsieh Wan Ling; Lai Chern Hsiung; Takeuchi Yasuo; Chen Yi Wen^*

来源：Carbohydrate Polymers, 2016, 151: 790-802.

DOI：10.1016/j.carbpol.2016.06.026

• Periodontitis
• Guided tissue regeneration
• Chitosan
• Collagen membrane
• Epigallocatechin-3-gallate
• Lovastatin

Currently used guided tissue regeneration (GTR) membranes are mainly used as a barrier to prevent epithelial cells growth into defects before new bone formation. The aim of this study was to develop a tri-layer functional chitosan (CS) membrane with epigallocatechin-3-gallate (EGCG) grafted on the outer layer for bactericidal activity, and lovastatin was included in the middle layer for controlled release. Successful EGCG grafting was demonstrated using Fourier transform infrared spectroscopy and EGCG grafting significantly enhanced adhesion and proliferation of human gingival fibroblasts. The release duration of lovastatin reached 21 days. CS-Lovastatin1 produced the highest alkaline phosphatase activity and EGCG14-CS exhibited the best bactericidal activity against periodontopathic bacteria. Finally, the EGCG14-CS-Lovastatini membrane showed a higher percentage of bone regeneration than BioMend and control groups in one -walled defects of beagle dogs. These results suggest that the EGCG14-CS-Lovastatini membrane has the potential to be used as a novel GTR membrane.

• 出版日期2016-10-20