Decontamination of contaminated soils by polycyclic aromatic hydrocarbons in the presence of supplementary organic structures

作者：Simeon Nathalie; Mercier Guy; Blais Jean Francois^*; Ouvrard Stephanie; Cebron Aurelie; Leyval Corinne; Goergen Jean Louis; Guedon Emmanuel

来源：Journal of Environmental Engineering and Science, 2008, 7(5): 467-479.

DOI：10.1139/S08-027

• polycyclic aromatic hydrocarbons (PAH)
• biodegradation
• contaminated soil
• sludge
• manure
• compost
• C/N ratio

Polycyclic aromatic hydrocarbons (PAHs) are well-known carcinogens and mutagens. In this study, the biodegradation of PAHs (16 major pollutants mentioned by the US Environmental Protection Agency) present in industrial polluted soil was carried out under addition of different organic substrates such as pig manure, shrimp compost, or sewage sludge. Wastewater sludge and pig manure were used as inoculum to enhance biodegradation of PAHs. The results showed a maximum biodegradation of 60% of total PAHs in 16aweeks at 30 degrees C. This degradation rate was obtained for a biopile that consisted of 75% of PAH contaminated soil, 10% of inoculum (acclimatized sludge), 10% of crude sludge, and 3% of straw (to increase aeration rate). The ratio of soil-amendment was 1.0:0.3 and the C/N ratio was 30. In this assay, GC-MS results showed a degradation of 68% +/- 16%.of PAH with five to six cycles. Quantification of PAH-dioxygenase genes in DNA extracted from soil and inoculum, using real-time polymerase chain reaction (RT-PCR) showed that the major bacterial degraders from the soil were Gram(+) bacteria, while in acclimated sludge (inoculum) major degraders were Gram(-) bacteria.

• 出版日期2008-9