Analysis of Annulated Sea Snake Venom, Hydrophis Cyanocinctus, Using Liquid Chromatography and MALDI-TOF/TOF

作者：Khorjestan Saeedeh Mosafer; Abtahi Behrooz; Siadat Seyed Omid Ranei; Motevalli Mohammad; Rezadoost Hassan; Ghezellou Parviz; Ghassempour Alireza^*

来源：Current Proteomics, 2015, 12(1): 45-55.

DOI：10.2174/157016461201150506201840

• Persian Gulf
• phospholipase A(2)
• sea snake
• three-finger toxin
• venom

Annulated sea snake, Hydrophis cyanocinctus, venom was investigated using reversed-phase high-performance liquid chromatography coupled to electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF). MALDI-TOF/TOF identified three compounds (a short-chain neurotoxin, a long-chain neurotoxin and a basic phospholipase A(2)); whose molecular weights were estimated to be 7285, 10838, and 14235 Da, respectively. Moreover, evolutionary relationship of each protein (identity more than 70%) was separately investigated via phylogenetic trees. This study was done as complementary investigation on the composition of the venom of H. cyanocinctus, the annulated sea snake from the Persian Gulf.

• 出版日期2015