Depletion of TGF-beta from fetal bovine serum

作者：Oida Takatoku; Weiner Howard L^*

来源：Journal of Immunological Methods, 2010, 362(1-2): 195-198.

DOI：10.1016/j.jim.2010.09.008

• TGF-beta
• LAP
• Fetal bovine serum
• Depletion
• Monoclonal antibody

TGF-beta is one of the key cytokines controlling immune responses. Measuring TGF-beta from culture supernatants in vitro is an important index of immune function. However, fetal bovine serum (FBS) contains a high level of latent TGF-beta that often hampers measuring T cell-derived TGF-beta in culture using FBS-supplemented medium. In this report, we generated anti-latency associated peptide (LAP) monoclonal antibodies which cross-react with bovine LAP, and developed a protocol to deplete TGF-beta from FBS. This provides the ability to reliably quantify TGF-13 in vitro without relying on serum-free media which do not support growth of murine T cells.

• 出版日期2010-10-31