Antinociceptive and anti-inflammatory effect of the Scutia buxifolia Reissek stem barks extract

作者：Moreira Lais da Rosa; Brum Evelyne da Silva; Haas da Silva Andreia Regina; de Freitas Mayara Lutchemeyer; Teixeira Taiane Piccini; Boligon Aline Augusti; Athayde Margareth Linde; Duarte Thiago; Medeiros Frescura Duarte Marta Maria; Oliveira Sara Marchesan; Brandao Ricardo^*

来源：Phytomedicine, 2016, 23(10): 1021-1028.

DOI：10.1016/j.phymed.2016.06.009

• Rhamnaceae
• Pain
• Inflammation
• Antinociception
• Scutia buxifolia

Background: Scutia buxifolia (Rhamnaceae) has been extensively studied for its phenolics groups, which are able to capture free radicals; being therefore, considered promising as an antioxidant in preventing diseases resulting from oxidative stress.
Hypothesis: Scutia buxifolia extract (SBE) presents antinociceptive and anti-inflammatory effect in mice.
Study Design: SBE (40 0-80 0 mg/kg) was tested in different pain models to investigate its antinociceptive and anti-inflammatory action.
Methods: It was carried out the abdominal writhing test, capsaicin test, thermal hyperalgesia and incisional pain. The inflamed tissue by carrageenan was used for the analysis of interleukins (IL), interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), c-reactive protein (CRP), nitrite and nitrate (NOx) determination and myeloperoxidase (MPO) activity. Furthermore, we evaluate the possible action mechanism of SBE using naloxone in capsaicin test.
Results: SBE prevented the nociception caused by acetic acid, formalin and capsaicin test. However, neither the SBE prevented the thermal hyperalgesia in hot-plate test, nor the naloxone reversed the SBE antinociceptive effect in capsaicin test. Furthermore, the administration of SBE prevented significantly the increase of MPO activity, the NOx content, and the levels of IL-1, IL-6, TNF-alpha, INF-gamma and CRP and was able to increase the IL-10 levels after the inflammation induced by carrageenan in mice. In addition, SBE prevented mechanical hyperalgesia in a postoperative pain model.
Conclusion: The SBE presents great antinociceptive and anti-inflammatory activity in mice but this effect not seem to have its action mechanism like opioids. It is possible that its antinociceptive effects are associated with levels decrease of inflammatory mediators.

• 出版日期2016-9-15