A Portable Liquid Chromatograph with a Battery-operated Compact Electroosmotic Pump and a Microfluidic Chip Device with a Reversed Phase Packed Column

作者：Ishida Akihiko; Fujii Mitsutaka; Fujimoto Takehiro; Sasaki Shunsuke; Yanagisawa Ichiro; Tani Hirofumi; Tokeshi Manabu

来源：Analytical Sciences, 2015, 31(11): 1163-1169.

DOI：10.2116/analsci.31.1163

• Electroosmotic pump
• liquid chromatography
• microfluidic chip
• packed column

A compact and lightweight liquid chromatography system is presented with overall dimensions of 26 cm width x 18 cm length x 21 cm height and weight of 2 kg. This system comprises a battery-operated compact electroosmotic pump, a manual injector, a microfluidic chip device containing a packed column and an electrochemical detector, and a USB bus-powered potentiostat. The pumping system was designed for microfluidic-based reversed-phase liquid chromatography in which an electroosmotically generated water stream pushes the mobile phase via a diaphragm for the output. The flow rate ranged from 0 to 10 mu L/min and had a high degree of precision. The pumping system operated continuously for over 24 h with dry batteries. The column formed in the microfluidic device was packed with 3-mu m ODS particles with a length of 30 mm and a diameter of 0.8 mm. The results presented herein demonstrate the performance of the pumping system and the column using alkylphenols, catecholamine, catechin, and amino acids.

• 出版日期2015-11